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| Bagheri,Samane; Gargari,Seyed Latif Mousavi; Rasooli,Iraj; Nazarian,Shahram; Alerasol,Masoome. |
| OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.METHODS: In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterotoxigenic Escherichia coli; CS6; Enterotoxin; Chimeric protein. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300308 |
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| Rodas,Claudia; Mamani,Rosalía; Blanco,Jorge; Blanco,Jesus Eulogio; Wiklund,Gudrun; Svennerholm,Ann-Mari; Sjöling,Åsa; Iniguez,Volga. |
| Enterotoxigenic Escherichia coli (ETEC) is recognized as the main cause of bacterial diarrhoea among children in Asia, Africa and Latin America but less investigated in Bolivia. OBJECTIVE: To determine the relation between enterotoxins, CFs and serotypes as well as the antimicrobial resistance patterns in a set of ETEC isolates collected from hospitalized children with acute diarrhea. In the present study we characterized 43 ETEC strains isolated from 2002 to 2006 from hospitalized children (0-5 years) with acute diarrhea in Bolivia. The strains were analyzed for heat-labile (LT) and heat-stable (ST) enterotoxins and colonization factor (CF) profiles, as well as for serogroups and antimicrobial resistance using phenotypic (ELISA, dot blot, slide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterotoxigenic Escherichia coli; Enterotoxins; Drug resistance; Bolivia. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200007 |
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| Figueiredo,H.C.P.; Lage,A.P.; Pereira Júnior,F.N.; Leite,R.C.. |
| A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals were allotted randomly to the blocks, each block consisting of two pregnant females (a vaccinated one and a control one) and their respective calves. In experiment A (farm 1), comprised of 18 blocks, the animals received a vaccine dose 30 days before delivery. In experiment B (farm 1), consisted of 26 blocks, the animals received two vaccine doses (60 and 30 days before delivery). In experiment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calf; Enterotoxigenic Escherichia coli; Vaccine; Diarrhea; K99; F41. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400001 |
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